Marks distribution: Total marks – 400

Category Details Marks Total Marks
Written MCQ (Single Best Answer + Multiple True Or False) 40 200
Short Answer Question (SAQ) + Structured Essay Question (SEQ) 140
Formative Assessment (FA) 20
SOE Structured Oral Examination 100 100
Practical Objective Structured Practical Exam (OSPE) 50 100
Traditional 40
Assignment 10
Grand Total 400

Learning Objectives and Course Contents in Biochemistry

Biophysics & Biomolecules


  • Introduction to Biochemistry
  • Concept of solutions
  • Colloids and crystalloids
  • Concept of isotope
  • Concept of Biomolecules: Carbohydrates
  • Amino acids and proteins
  • Lipids and fatty acids
  • Enzymes


  • Basic concepts of food, nutrition, and dietary principles
  • Energy balance and calculation of energy equivalent of food
  • Nutritional aspects of carbohydrates, fats and proteins, Fibers
  • Vitamins and Minerals
  • Common Nutritional Disorders


Digestion, Absorption, Bioenergetics and Metabolism


  • Introduction to Metabolism
  • Biological oxidation, respiratory chain, and oxidative phosphorylation
  • High and low energy compounds. ATP
  • The phase of metabolism (digestion, absorption, and intermediary metabolism)
  • Glycolysis
  • Citric acid cycle
  • Glycogenesis and glycogenolysis
  • Hexose monophosphate shunt
  • Gluconeogenesis
  • Blood glucose homeostasis
  • Cori cycle

Lipid Metabolism


  • Digestion and absorption of lipid
  • Blood lipids and pathways of lipid metabolism
  • Triglyceride metabolism
  • Beta-oxidation
  • Ketogenesis and ketosis
  • Lipid transport and lipoprotein metabolism
  • Ecosanoids

Protein Metabolism


  • Digestion and absorption of protein
  • Protein turnover, common amino acid pool, nitrogen balance
  • Pathways of protein metabolism
  • Deamination and transamination
  • Fate of amino acid in the body
  • Source and disposal of ammonia


  • Role of liver in over all metabolisms
  • Integrated metabolism
  • Metabolic adjustment of fed, fasting and starvation state


Renal biochemistry, body fluid, electrolytes and acid-base balance


Renal biochemistry in relation to water, electrolytes and acid base homeostasis

  • Total body water and body fluid compartments. Composition of body fluids
  • Regulation of normal water balance
  • Major electrolytes and their homeostasis
  • Volume disorders
  • Acid base homeostasis & disorders


Clinical Biochemistry and clinical endocrinology


  • Introduction to clinical biochemistry
  • Normal biochemical values in conventional and Sl. Units
  • Clinical enzymology related to liver and myocardial diseases
  • Lipid profiles and dyslipoproteinemias
  • Organ function tests (liver, kidney & thyroid)
  • Diagnosis of diabetes mellitus
  • Bilirubin metabolism and Jaundice
  • Proteinuria and microalbuminuria


Fundamentals of Molecular Biology and genetics


  • Basic concepts of molecular biology
  • Nnucleic acid, nucleosides, and nucleotides
  • Replication, transcription and translation
  • Gene, genome, allele, trait, genetic code, mutation, mutagens
  • PCR, DNA cloning, recombinant DNA technology
  • Biomedical aspects of medical biotechnology: understanding & application


Biochemistry practical


  • Identification of laboratory glass wares and equipment
  • Preparation of solutions
  • Sample collection & processing
  • Photometry
  • Estimation, demonstration of technique, calculation and interpretation of result
  • Blood glucose estimation
  • Serum cholesterol estimation
  • Serum urea
  • Serum creatinine
  • Serum total protein
  • Serum bilirubin
  • Abnormal constituents of urine and their clinical significance